Silicone ring implantation in an excisional murine wound model.

UNLABELLED A good animal model for wound healing is indispensable for researchers to study the basic mechanism of tissue repair, and to develop strategies for clinical treatment. Small mammalian wound healing models are the most popular animal models for wound healing research because they are inexpensive, readily obtainable, and easy to handle. One significant challenge of using mice to evaluate wound repair is that wound contraction originates outside of tissue, whereas in humans, re-epithelialization and granulation tissue formation occurs within the wound space. METHODS The present study describes a new excisional skin wound model utilizing an implanted silicone ring on the dorsal side of the mouse for 1 week prior to creating a fullthickness skin defect wound. RESULTS The results showed that the time required for complete epithelialization of the wound was extended, the re epithelialization ratio was increased, and more granulation tissue was formed. CONCLUSION Permitting the wound to heal mainly through re-epithelialization and granulation tissue formation, this new technique can result in a new excisional murine wound model that closer approximates human wound healing, allowing for more relevant evaluation of molecular signaling and cellular metabolism that occur during skin wound healing. .